UCHL5 Polyklonaler Antikörper

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UCHL5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11527-1-PBS

Synonyme

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  WB analysis of mouse colon using 11527-1-AP (same clone as 11527-1-PBS)

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11527-1-AP (same clone as 11527-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11527-1-AP (UCHL5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 11527-1-AP (same clone as 11527-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11527-1-AP (UCHL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11527-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11527-1-PBS bindet in WB, IHC, Indirect ELISA UCHL5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UCHL5 fusion protein Ag2095
• Vollständiger Name: ubiquitin carboxyl-terminal hydrolase L5
• Berechnetes Molekulargewicht: 326 aa, 37 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC025369
• Gene symbol: UCHL5
• Gene ID (NCBI): 51377
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCHL5 (Ubiquitin carboxyl-terminal hydrolase isozyme L5) is also named as UCH37 and belongs to the peptidase C12 family. It is functionally linked with multiple protein complexes and signal transduction pathways. UCH37's weight average molecular weight increases as a function of its concentration. By size-exclusion chromatography, the apparent molecular weight of UCH37 was 51, 56, 70, and 109 kDa at 0.15, 0.69, 2.4, and 9.5 mg/mL, respectively. Given UCH37's monomeric molecular weight of 37 kDa and its apparent elongated shape, its tendency toward an apparent molecular weight between 40 and 50 kDa at decreasing concentrations is appropriate for UCH37 monomers (PMID:21953935).