UCKL1 Polyklonaler Antikörper

UCKL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 17005-1-AP

Synonyme

F538, UCK1 LIKE, UCK1L, UCKL1, Uridine cytidine kinase like 1, URKL1

---

• 

  WB analysis of K-562 using 17005-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 17005-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 17005-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 17005-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 17005-1-AP (UCKL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17005-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17005-1-AP (UCKL1 antibody) at dilution of 1:100 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Raji-Zellen, Rattennierengewebe, U-937-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Hirngewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

17005-1-AP bindet in WB, IHC, ELISA UCKL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UCKL1 fusion protein Ag10701
• Vollständiger Name: uridine-cytidine kinase 1-like 1
• Berechnetes Molekulargewicht: 419 aa, 46 kDa
• Beobachtetes Molekulargewicht: 61 kDa, 46 kDa
• GenBank-Zugangsnummer: BC033078
• Gene symbol: UCKL1
• Gene ID (NCBI): 54963
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB,IHC	EBioMedicine; Non-canonical role of UCKL1 on ferroptosis defence in colorectal cancer; Authors - Weili Wu ; KD Validated