UCKL1 Polyklonaler Antikörper

UCKL1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28762-1-PBS

Synonyme

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  WB analysis using 28762-1-AP (same clone as 28762-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28762-1-AP (UCKL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28762-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 28762-1-AP (same clone as 28762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28762-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28762-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 28762-1-AP (same clone as 28762-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28762-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28762-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28762-1-PBS bindet in WB, IHC, Indirect ELISA UCKL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UCKL1 fusion protein Ag30535
• Vollständiger Name: uridine-cytidine kinase 1-like 1
• Berechnetes Molekulargewicht: 419 aa, 46 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC033078
• Gene symbol: UCKL1
• Gene ID (NCBI): 54963
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uridine cytidine kinase like-1 (UCKL-1) is a largely uncharacterized protein over-expressed in many tumor cells, especially in highly malignant, aggressive tumors. Sequence analysis indicates that UCKL-1 has homology to uridine kinases, enzymes that play a role in DNA and RNA synthesis and that are often up-regulated in tumor cells. Previous studies have shown that UCKL-1 is a substrate for natural killer lytic-associated molecule (NKLAM), an E3 ubiquitin ligase found in NK cell cytolytic granules. Ubiquitination of UCKL-1 by NKLAM leads to its degradation. Increased expression of NKLAM enhances NK-mediated tumoricidal activity. (PMID: 32083188)