Urocortin Rekombinanter Antikörper

Urocortin Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241183B12

Kat-Nr. : 84380-1-PBS

Synonyme

UCN, UROC, UI, 241183B12

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  WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 84380-1-RR (same clone as 84380-1-PBS)

  Rat kidney tissue were subjected to SDS PAGE followed by western blot with 84380-1-RR (UCN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 84380-1-RR (same clone as 84380-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 84380-1-RR (Urocortin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84380-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84380-1-PBS bindet in WB, IHC, Indirect ELISA Urocortin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: urocortin
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: NM_003353
• Gene symbol: UCN
• Gene ID (NCBI): 7349
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Urocortin is a member of the sauvagine/corticotropin-releasing factor/urotensin I family that includes CRF, urotensin I, sauvagine, urocortin II and urocortin III. Urocortin is a potent anorexigenic peptide that induces fed-like motor activity when administered centrally or peripherally in fasted animals. Urocortin is also a potent and long-lasting hypotensive agent and increases coronary blood flow. Urocortin acts in vitro to stimulate the secretion of adrenocorticotropic hormone (ACTH) and binds with high affinity to CRF receptor types 1, 2-alpha, and 2-beta. Urocortin is synthesized as precursor, which is subsequently processed to the mature biologically active peptide (a 40-amino acid Molecule ). The 22 kDa may be a urocortin 1 precursor protein (PMID: 28887029).