UCP1 Polyklonaler Antikörper

UCP1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

79

Rezensionen (1)

Kat-Nr. : 23673-1-AP

Synonyme

SLC25A7, Thermogenin, UCP, UCP 1, UCP1

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Galerie der Validierungsdaten

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  WB analysis using 23673-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23673-1-AP (UCP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brown adipose using 23673-1-AP

  mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 23673-1-AP (UCP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 23673-1-AP (UCP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat brain using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 23673-1-AP (UCP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brown adipose using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 23673-1-AP (UCP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 23673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 23673-1-AP (UCP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: braunes Mausfettgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: braunes Mausfettgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 68 publications below
• IHC: See 23 publications below
• IF: See 7 publications below

Produktinformation

23673-1-AP bindet in WB, IHC, IF, ELISA UCP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UCP1 fusion protein Ag20388
• Vollständiger Name: uncoupling protein 1 (mitochondrial, proton carrier)
• Berechnetes Molekulargewicht: 307 aa, 33 kDa
• Beobachtetes Molekulargewicht: 30-33 kDa
• GenBank-Zugangsnummer: BC098168
• Gene symbol: UCP1
• Gene ID (NCBI): 7350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.

Publikationen

Species	Application	Title
mouse	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu ; KO Validated
mouse	WB	Nat Commun; Improvement of obesity-associated disorders by a small-molecule drug targeting mitochondria of adipose tissue macrophages.; Authors - Yawei Wang
mouse	WB,IHC	Mol Biol Evol; Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans; Authors - Ming Zhou
mouse	WB	Diabetes; Exosomes From Adipose-Derived Stem Cells Attenuate Adipose Inflammation and Obesity Through Polarizing M2 Macrophages and Beiging in White Adipose Tissues.; Authors - Hui Zhao
mouse	WB	JCI Insight; Muscle specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolism; Authors - Benedict Abdon
	WB	Diabetes; PMID: 23990362; Authors - Wang Qun Q

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mi (Verified Customer) (03-06-2023)

It works well in human brown adipocyte cells. Although we got several non-specific bands, the band at the expected size was very clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: human brown adipocytes

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