UCP1 Rekombinanter Antikörper

UCP1 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240782F3

Kat-Nr. : 83870-1-PBS

Synonyme

240782F3, SLC25A7, Thermogenin, UCP, UCP 1

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  WB analysis of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

  mouse brown adipose tissue were subjected to SDS PAGE followed by western blot with 83870-1-RR (UCP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 83870-1-RR (same clone as 83870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 83870-1-RR (same clone as 83870-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83870-1-RR (UCP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brown adipose using 83870-1-RR (same clone as 83870-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brown adipose tissue using UCP1 antibody (83870-1-RR, Clone: 240782F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83870-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83870-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83870-1-RR against Human UCP1 were performed. The affinity constant is 64.6 nM.

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Geprüfte Anwendungen

Produktinformation

83870-1-PBS bindet in WB, IHC, IF-P, ELISA UCP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UCP1 fusion protein Ag20388
• Vollständiger Name: uncoupling protein 1 (mitochondrial, proton carrier)
• Berechnetes Molekulargewicht: 307 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC098168
• Gene symbol: UCP1
• Gene ID (NCBI): 7350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone.