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UCP2 Monoklonaler Antikörper

UCP2 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG3

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B4B1

Kat-Nr. : 66700-1-PBS

Synonyme

2B4B1, BMIQ4, Dicarboxylate carrier SLC25A8, SLC25A8, UCP 2

Filter:
  • Western Blot (WB) analysis of Jurkat cells using UCP2 Monoclonal antibody (66700-1-Ig)

    WB analysis of Jurkat using 66700-1-Ig (same clone as 66700-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adipose tissue using UCP2 Monoclonal antibody (66700-1-Ig)

    WB analysis of human adipose using 66700-1-Ig (same clone as 66700-1-PBS)

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using UCP2 Monoclonal antibody (66700-1-Ig)

    WB analysis of human skeletal muscle using 66700-1-Ig (same clone as 66700-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66700-1-Ig (UCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66700-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66700-1-PBS bindet in WB, Indirect ELISA UCP2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG3
Klonalität Monoklonal
Typ Antikörper
Immunogen UCP2 fusion protein Ag19361
Vollständiger Name uncoupling protein 2 (mitochondrial, proton carrier)
Berechnetes Molekulargewicht 33 kDa
Beobachtetes Molekulargewicht 28 kDa
GenBank-ZugangsnummerBC011737
Gene symbol UCP2
Gene ID (NCBI) 7351
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.