UFC1 Polyklonaler Antikörper

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• KD/KO Validated

UFC1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 15783-1-AP

Synonyme

HSPC155, UFC1, Ufm1 conjugating enzyme 1

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  WB analysis using 15783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15783-1-AP (UFC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15783-1-AP

  WB result of UFC1 antibody (15783-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UFC1 transfected A549 cells.

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  IHC staining of human liver using 15783-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15783-1-AP (UFC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15783-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15783-1-AP (UFC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, MCF-7-Zellen, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

15783-1-AP bindet in WB, IHC, IF, IP, ELISA UFC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UFC1 fusion protein Ag8480
• Vollständiger Name: ubiquitin-fold modifier conjugating enzyme 1
• Berechnetes Molekulargewicht: 167 aa, 19 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC005187
• Gene symbol: UFC1
• Gene ID (NCBI): 51506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The function of UFC1 (ubiquitin fold modifier conjugating enzyme 1) has not been widely studied, and is yet to be fully elucidated. The MW of this protein is 21 kDa, and this antibody specially recognises the 21 kDa protein. Catalog#15783-1-AP specially recognises the 21 kDa protein.

Publikationen

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Mol Cell; UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity; Authors - Chuan He ; KD Validated
human	WB	J Biol Chem; Mechanistic Study of Uba5 and the Ufm1 Conjugation Pathway.; Authors - James M Gavin
		J Cell Sci; ACSL3 is a novel GABARAPL2 interactor that links ufmylation and lipid droplet biogenesis.; Authors - Franziska Eck
	WB	Cells; Ufmylation of UFBP1 Is Dispensable for Endoplasmic Reticulum Stress Response, Embryonic Development, and Cardiac and Intestinal Homeostasis; Authors - Varsha Tandra
	IF	Medicines (Basel); Hypomyelinating Leukodystrophy 14 (HLD14)-Related UFC1 p.Arg23Gln Decreases Cell Morphogenesis: A Phenotype Reversable with Hesperetin; Authors - Yuri Ichihara