UFM1 Polyklonaler Antikörper

UFM1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 15883-1-AP

Synonyme

BM 002, C13orf20, ubiquitin fold modifier 1, UFM1

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 15883-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15883-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15883-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15883-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15883-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15883-1-AP (UFM1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15883-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15883-1-AP (UFM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HEK-293-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Mauslungengewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15883-1-AP bindet in WB, IHC, ELISA UFM1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UFM1 fusion protein Ag8667
• Vollständiger Name: ubiquitin-fold modifier 1
• Berechnetes Molekulargewicht: 85 aa, 9 kDa
• Beobachtetes Molekulargewicht: 9-12 kDa
• GenBank-Zugangsnummer: BC005193
• Gene symbol: UFM1
• Gene ID (NCBI): 51569
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UFM1, also named as C13orf20, is a ubiquitin-like modifier protein which binds to a number of target proteins, such as DDRGK1.

Publikationen

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu