UFSP2 Polyklonaler Antikörper

UFSP2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 16999-1-AP

Synonyme

Ufm1-specific protease 2, Ufm1 specific protease 2, EC:3.4.22.-, C4orf20

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  WB analysis of mouse pancreas using 16999-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16999-1-AP (UFSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16999-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16999-1-AP (UFSP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16999-1-AP

  IP result of anti-UFSP2 (IP:16999-1-AP, 3ug; Detection:16999-1-AP 1:1000) with HepG2 cells lysate 1700ug.

• 

  IHC staining of human liver using 16999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16999-1-AP (UFSP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16999-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16999-1-AP (UFSP2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

16999-1-AP bindet in WB, IHC, IP, ELISA UFSP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UFSP2 fusion protein Ag10692
• Vollständiger Name: UFM1-specific peptidase 2
• Berechnetes Molekulargewicht: 469 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC010493
• Gene symbol: UFSP2
• Gene ID (NCBI): 55325
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin-fold modifier 1 (Ufm1)-specific protease 2 (UfSP2) is a cysteine protease that is responsible for the release of Ufm1 from Ufm1-conjugated cellular proteins, as well as for the generation of mature Ufm1 from its precursor (PMID: 21228277). The MW of this protein is 53 kDa, and this antibody specially recognises the 53 kDa protein.

Publikationen

Species	Application	Title
human,mouse	WB	Genet Med; A pathogenic UFSP2 variant in an autosomal recessive form of pediatric neurodevelopmental anomalies and epilepsy.; Authors - Min Ni
		FASEB J; UFMylation of HRD1 regulates endoplasmic reticulum homeostasis; Authors - Hui Luo
	WB	Cells; Ufmylation of UFBP1 Is Dispensable for Endoplasmic Reticulum Stress Response, Embryonic Development, and Cardiac and Intestinal Homeostasis; Authors - Varsha Tandra
human	WB	Sci Adv; The ufmylation cascade controls COPII recruitment, anterograde transport, and sorting of nascent GPCRs at ER; Authors - Xin Xu ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Isabelle Cristine (Verified Customer) (01-14-2022)

This antibody works extremely well and is very specific for detecting the UFSP2 protein in HEK293 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

UFSP2 Antibody Western Blot validation (1:1000 dilution) in HEK293 (Cat no:16999-1-AP)