UGCGL1 Polyklonaler Antikörper

UGCGL1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 14170-1-AP

Synonyme

UGGT1, UDP-glucose:glycoprotein glucosyltransferase 1, UDP-glucose ceramide glucosyltransferase-like 1, UDP--Glc:glycoprotein glucosyltransferase, hUGT1

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  WB analysis using 14170-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14170-1-AP (UGCGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14170-1-AP

  IP result of anti-UGCGL1 (IP:14170-1-AP, 5ug; Detection:14170-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 14170-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 14170-1-AP (UGCGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14170-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14170-1-AP (UGCGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14170-1-AP bindet in WB, IHC, IF, IP, ELISA UGCGL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UGCGL1 fusion protein Ag5369
• Vollständiger Name: UDP-glucose ceramide glucosyltransferase-like 1
• Berechnetes Molekulargewicht: 1531 aa, 175 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC041098
• Gene symbol: UGCGL1
• Gene ID (NCBI): 56886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UDP-glucose:glycoprotein glucosyltransferase 1 (UGCGL1) is a central quality control gatekeeper in the mammalian endoplasmic reticulum (ER) and serves as a folding sensor in the calnexin/calreticulin glycoprotein quality control cycle (PMID: 28425917). UGCGL1 acts as a checkpoint in the quality control of the MHC class I antigen presentation pathway (PMID: 21383159). Western blot analysis detected UGCGL1 at an apparent molecular mass of 170 kDa.

Publikationen

Species	Application	Title
rat	WB	J Cell Biol; Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins; Authors - Amber M Cheatham
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright
human	WB	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis.; Authors - Madison T Wright
rat	IF,WB	Toxicology; Tauroursodeoxycholic acid attenuates cisplatin-induced ototoxicity by inhibiting the accumulation and aggregation of unfolded or misfolded proteins in the endoplasmic reticulum.; Authors - Yingying Wen
human	WB	Glycobiology; Polypeptide N-acetylgalactosaminyltransferase 18 Retains in Endoplasmic Reticulum Depending on its Luminal Regions Interacting with ER resident UGGT1, PLOD3 and LPCAT1.; Authors - Wenjuan Jia
mouse	WB	Biochem Biophys Res Commun; UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner.; Authors - Jaeyong Cho