UGDH Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UGDH Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 13151-1-AP

Synonyme

UDP-glucose 6-dehydrogenase, UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH

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  WB analysis using 13151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 13151-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13151-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13151-1-AP (UGDH Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 13151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13151-1-AP (UGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13151-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13151-1-AP (UGDH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13151-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using UGDH antibody (13151-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HepG2-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 3 publications below

Produktinformation

13151-1-AP bindet in WB, IHC, IF/ICC, ELISA UGDH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UGDH fusion protein Ag3767
• Vollständiger Name: UDP-glucose dehydrogenase
• Berechnetes Molekulargewicht: 494 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC022781
• Gene symbol: UGDH
• Gene ID (NCBI): 7358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

Publikationen

Species	Application	Title
human	WB	Nature; UDP-glucose accelerates SNAI1 mRNA decay and impairs lung cancer metastasis.; Authors - Xiongjun Wang ; KD Validated
human	WB, IHC	Arthritis Res Ther; UDP-glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis.; Authors - Yinxian Wen ; KD Validated
human	WB	PLoS One; Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways.; Authors - Qi Ying
human	WB, IHC	PLoS One; Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.; Authors - Yinxian Wen
human,mouse	WB	Nat Commun; UDP-glucuronate metabolism controls RIPK1-driven liver damage in nonalcoholic steatohepatitis; Authors - Tao Zhang
human	WB,IHC	Arch Biochem Biophys; UGDH promotes 5-fluorouracil resistance in colorectal cancer via the ROS-activated PI3K/AKT-EEF1A2-PRDX1 pathway; Authors - Jinfeng Cai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain