UGDH Monoklonaler Antikörper

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• KD/KO Validated

UGDH Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1E2E1

Kat-Nr. : 67360-1-Ig

Synonyme

UDP-GlcDH, UDP-Glc dehydrogenase, UDPGDH, GDH, EC:1.1.1.22

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  WB analysis using 67360-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 67360-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67360-1-Ig (UGDH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67360-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67360-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67360-1-Ig (UGDH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67360-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UGDH antibody (67360-1-Ig, Clone: 1E2E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, HepG2-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

67360-1-Ig bindet in WB, IHC, IF/ICC, IP, ELISA UGDH und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UGDH fusion protein Ag29747
• Vollständiger Name: UDP-glucose dehydrogenase
• Berechnetes Molekulargewicht: 494 aa, 55 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC022781
• Gene symbol: UGDH
• Gene ID (NCBI): 7358
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UDP-glucose 6-dehydrogenase (UGDH) is a key enzyme in the uronic acid pathway, and converts UDP-glucose (UDP-Glc) to UDP-glucuronic acid (UDP-GlcA). UGDH is critical to the production of extracellular matrix components which are essential to the migration and connectivity of neurons early in human brain development. UDP-GlcA is an essential sugar nucleotide precursor and a key component in the synthesis and stepwise degradation of glycosaminoglycans (GAGs). The protein subunit migrates on SDS-PAGE at ~55 kDa. (PMID: 32175296, PMID: 21576248, PMID: 31243371).

Publikationen

Species	Application	Title
human,mouse	WB	Biochem Biophys Res Commun; UDP-glucose 6-dehydrogenase lessens sorafenib sensitivity via modulating unfolded protein response.; Authors - Bao Guo ; KO Validated
human	WB,IP,IF	Adv Sci (Weinh); UGDH Lactylation Aggravates Osteoarthritis by Suppressing Glycosaminoglycan Synthesis and Orchestrating Nucleocytoplasmic Transport to Activate MAPK Signaling; Authors - Weiren Lan