UGP2 Polyklonaler Antikörper

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• KD/KO Validated

UGP2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10391-1-AP

Synonyme

pHC379, UDP glucose pyrophosphorylase, UDPG, UDPGP, UDPGP2, UGP1, UGP2, UGPase, UGPP2

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Galerie der Validierungsdaten

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  WB analysis using 10391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10391-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10391-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10391-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10391-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10391-1-AP (UGP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10391-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10391-1-AP (UGP2 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human liver cancer using 10391-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10391-1-AP (UGP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10391-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10391-1-AP (UGP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Produktinformation

10391-1-AP bindet in WB, IHC, ELISA UGP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UGP2 fusion protein Ag0590
• Vollständiger Name: UDP-glucose pyrophosphorylase 2
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 50-56 kDa
• GenBank-Zugangsnummer: BC002954
• Gene symbol: UGP2
• Gene ID (NCBI): 7360
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UDP-glucose pyrophosphorylase 2 (UGP2, synonyms: UDPG, UGPP2, UDPGP2, Phc379)is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. So far two isoforms are encoded by two transcript variants have been found.

Publikationen

Species	Application	Title
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding
rat	IHC	Toxicol Sci; Mitochondrial prohibitins and septin 9 are implicated in the onset of rat hepatocarcinogenesis.; Authors - Kakehashi Anna A
human	IHC	Dis Markers; Low UGP2 Expression Is Associated with Tumour Progression and Predicts Poor Prognosis in Hepatocellular Carcinoma.; Authors - Qiuyue Hu
human	WB	J Cell Biochem; Identification of UGP2 as a progression marker that promotes cell growth and motility in human glioma.; Authors - Chun Zeng ; KD Validated