UGT8 Polyklonaler Antikörper

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• KD/KO Validated

UGT8 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17982-1-PBS

Synonyme

UGT 8, UDP-galactose-ceramide galactosyltransferase, EC:2.4.1.47, CGT, Ceramide UDP-galactosyltransferase

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  WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17982-1-AP (UGT8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

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  IP experiment of rat brain using 17982-1-AP (same clone as 17982-1-PBS)

  IP result of anti-UGT8 (IP:17982-1-AP, 4ug; Detection:17982-1-AP 1:1000) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 17982-1-AP (same clone as 17982-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17982-1-AP (UGT8 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17982-1-AP (same clone as 17982-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17982-1-AP (same clone as 17982-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17982-1-AP (UGT8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17982-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17982-1-PBS bindet in WB, IHC, IP, Indirect ELISA UGT8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UGT8 fusion protein Ag12393
• Vollständiger Name: UDP glycosyltransferase 8
• Berechnetes Molekulargewicht: 541 aa, 61 kDa
• Beobachtetes Molekulargewicht: 61 kDa
• GenBank-Zugangsnummer: BC075069
• Gene symbol: UGT8
• Gene ID (NCBI): 7368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells. This antibody specifically recognizes the endogenous UGT8.