UHMK1 Polyklonaler Antikörper

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• KD/KO Validated

UHMK1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11624-1-AP

Synonyme

KIS, Kist, U2AF homology motif kinase 1, UHMK1

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Galerie der Validierungsdaten

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  WB analysis of mouse thymus using 11624-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11624-1-AP (UHMK1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11624-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11624-1-AP (UHMK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11624-1-AP

  IP Result of anti-UHMK1 (IP:11624-1-AP, 4ug; Detection:11624-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human stomach using 11624-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11624-1-AP (UHMK1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 11624-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11624-1-AP (UHMK1 antibody) at dilution of 1:200 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

11624-1-AP bindet in WB, IP, IHC, IF, ELISA UHMK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UHMK1 fusion protein Ag2204
• Vollständiger Name: U2AF homology motif (UHM) kinase 1
• Berechnetes Molekulargewicht: 419 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC026046
• Gene symbol: UHMK1
• Gene ID (NCBI): 127933
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UHMK1, also named as KIS, or KIST, is a 419 amino acid protein, which contains one RRM (RNA recognition motif) domain, one protein kinase domain, and belongs to the protein kinase superfamily. UHMK1 is widely expressed, with highest levels in skeletal muscle, kidney, placenta and peripheral blood leukocytes. UHMK1 localizes in the nucleus and upon serum stimulation, phosphorylating CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. UHMK1 may be involved in trafficking and/or processing of RNA and may have a role in the developing nervous system and the adult brain.

Publikationen

Species	Application	Title
rat	IHC	Brain Res; Cord blood administration induces oligodendrocyte survival through alterations in gene expression.; Authors - Rowe D D DD
human	WB,IHC,IF	Cell Death Dis; UHMK1 aids colorectal cancer cell proliferation and chemoresistance through augmenting IL-6/STAT3 signaling.; Authors - Xuedi Gao ; KD Validated
human	WB	Thorac Cancer; UHMK1 promotes lung adenocarcinoma oncogenesis by regulating the PI3K/AKT/mTOR signaling pathway; Authors - Yongmeng Li ; KD Validated