UHRF1 Polyklonaler Antikörper

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• KD/KO Validated

UHRF1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21402-1-PBS

Synonyme

ICBP90, HuNp95, hUHRF1, hNp95, EC:2.3.2.27

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  WB analysis using 21402-1-AP (same clone as 21402-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21402-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 21402-1-AP (same clone as 21402-1-PBS)

  WB result of UHRF1 antibody (21402-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UHRF1 transfected HEK-293T cells. This data was developed using the same antibody clone with 21402-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21402-1-AP (same clone as 21402-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21402-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 21402-1-AP (same clone as 21402-1-PBS)

  IP result of anti-UHRF1 (IP:21402-1-AP, 4ug; Detection:21402-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 21402-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 21402-1-AP (same clone as 21402-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (21402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21402-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21402-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA UHRF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UHRF1 fusion protein Ag16111
• Vollständiger Name: ubiquitin-like with PHD and ring finger domains 1
• Berechnetes Molekulargewicht: 806 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91-100 kDa
• GenBank-Zugangsnummer: BC113875
• Gene symbol: UHRF1
• Gene ID (NCBI): 29128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).