UHRF1 Rekombinanter Antikörper

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• KD/KO Validated

UHRF1 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241872B12

Kat-Nr. : 84489-4-RR

Synonyme

241872B12, E3 ubiquitin-protein ligase UHRF1, EC:2.3.2.27, hNp95, hUHRF1

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  WB analysis using 84489-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84489-4-RR (UHRF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 84489-4-RR

  WB result of UHRF1 antibody (84489-4-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UHRF1 transfected HEK-293T cells.

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  IF Staining of HepG2 using 84489-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (84489-4-RR, Clone: 241872B12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U2OS using 84489-4-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug UHRF1 Recombinant antibody (84489-4-RR, Clone:241872B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen, Jurkat-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84489-4-RR bindet in WB, IF/ICC, FC (Intra), ELISA UHRF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UHRF1 fusion protein Ag17498
• Vollständiger Name: ubiquitin-like with PHD and ring finger domains 1
• Berechnetes Molekulargewicht: 806 aa, 91 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC113875
• Gene symbol: UHRF1
• Gene ID (NCBI): 29128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).