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UHRF2 Polyklonaler Antikörper

UHRF2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25710-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using UHRF2 Polyclonal antibody (25710-1-AP)

    WB analysis using 25710-1-AP (same clone as 25710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25710-1-AP (UHRF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25710-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using UHRF2 Polyclonal antibody (25710-1-AP)

    IP experiment of Jurkat using 25710-1-AP (same clone as 25710-1-PBS)

    IP result of anti-UHRF2 (IP:25710-1-AP, 4ug; Detection:25710-1-AP 1:300) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 25710-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UHRF2 Polyclonal antibody (25710-1-AP)

    IF Staining of HepG2 using 25710-1-AP (same clone as 25710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF2 antibody (25710-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25710-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25710-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA UHRF2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen UHRF2 fusion protein Ag22519
Vollständiger Name ubiquitin-like with PHD and ring finger domains 2
Berechnetes Molekulargewicht 90 kDa
Beobachtetes Molekulargewicht90 kDa
GenBank-ZugangsnummerBC028397
Gene symbol UHRF2
Gene ID (NCBI) 115426
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ubiquitin-Like with PHD and ring finger domains 2 (UHRF2), a member that belongs to the family of UHRF, contains five recognizable functional domains, namely the ubiquitin-like domain (UBL) domain, tandem-Tudor domain (TTD), plant homeodomain (PHD), SET and RING associated (SRA) domain, and really interesting new gene (RING) finger domain. Due to the complex structure, UHRF2 possesses multiple functions in diverse cellular processes. As a ubiquitin E3 ligase, UHRF2 could ubiquitinate PCNP, a nuclear protein that contains two remarkable PEST sequences which are rich in proline (P), glutamic acid (E), serine (S), and threonine (T). It has been also reported that UHRF2 could serve as a vital cell cycle regulator by interacting with multiple cyclins, CDKs, p53, pRB and PCNA. UHRF2 has been revealed to possess epigenetic regulation function and is capable of maintaining 5mC levels in certain genomic loci in brain and stabilizes TIP60 to regulate H3K9ac and H3K14ac through RING finger domain. Moreover, UHRF2 could promote DNA damage repair by reducing the level of p21 mediated by RING finger domain. Recently, emerging evidence indicated that UHRF2 was involved in the tumorigenesis and progression of several human cancers, such as esophageal squamous cell carcinoma, lung cancer and colorectal cancer. UHRF2 has 2 isoforms with the molecular mass of 56 and 90 kDa. (PMID: 34400880)