ULBP2 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

ULBP2 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240413A6

Kat-Nr. : 83709-1-RR

Synonyme

240413A6, ALCAN alpha, ALCAN-alpha, N2DL2, N2DL-2

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  WB analysis of Jurkat using 83709-1-RR

  WB result of ULBP2 antibody (83709-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ULBP2 transfected Jurkat cells.

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  WB analysis using 83709-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83709-1-RR (ULBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 83709-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 83709-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83709-1-RR (ULBP2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83709-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83709-1-RR against Human ULBP2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, COLO 320-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83709-1-RR bindet in WB, IHC, ELISA ULBP2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ULBP2 fusion protein Eg0858
• Vollständiger Name: UL16 binding protein 2
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: NM_025217.4
• Gene symbol: ULBP2
• Gene ID (NCBI): 80328
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NKG2D is an activating cell surface receptor that is predominantly expressed on cytotoxic immune cells. NKG2D recognizes a wide range of ligands. In humans, the NKG2D ligand (NKG2DL) includes MICA, MICB, and six members of the ULBP family (ULBP1-6) (PMID: 29568297; 30813924). ULBPs are MHC class I-related molecules (PMID: 11239445). ULBP2 contains two Ig-like domains, the alpha-1 and alpha-2 domains characteristic of the MHC class I family, but lacks the alpha-3 domain. It can be expressed as either a transmembrane or GPI-linked protein, or released from the cell surface (PMID: 21224393; 24614922). ULBP2 binds and activates the NKG2D, mediating the recruitment and activation of NK cells.