ULK1 Polyklonaler Antikörper

ULK1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Kat-Nr. : 29005-1-AP

Synonyme

ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

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  WB analysis of HeLa using 29005-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 29005-1-AP (ULK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29005-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ULK1 antibody (29005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

29005-1-AP bindet in WB, IHC, IF/ICC, ELISA ULK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-51-like kinase 1 (C. elegans)
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: NM_003565
• Gene symbol: ULK1
• Gene ID (NCBI): 8408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).

Publikationen

Species	Application	Title
goat	WB	Ecotoxicol Environ Saf; Enhanced autophagy reversed aflatoxin B1-induced decrease in lactate secretion of dairy goat Sertoli cells; Authors - Shuaiqi Han
human	WB	Sci Rep; EYA3 promotes the tumorigenesis of gastric cancer through activation of the mTORC1 signaling pathway and inhibition of autophagy; Authors - Zhen Xu
human	WB	Cytojournal; Exosomes derived from cancer-associated fibrolasts mediated ciplatin resistance; Authors - Yiyu Meng
mouse	WB	Sci China Life Sci; GLA deficiency causes cardiac hypertrophy via enhanced autophagy; Authors - Wei Ou
mouse	WB,IHC,IF	Aging Cell; DLK/JNK3 Upregulation Aggravates Hair Cell Senescence in Mice Cochleae via Excessive Autophagy; Authors - Rui Ding
human		Med Oncol; GNL3L promotes autophagy via regulating AMPK signaling in esophageal cancer cells; Authors - Weiting He