ULK1 Monoklonaler Antikörper

ULK1 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D7G1

Kat-Nr. : 68445-1-Ig

Synonyme

ATG1, FLJ38455, FLJ46475, KIAA0722, ULK1, Unc 51 like kinase 1, UNC51, Unc51.1

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Galerie der Validierungsdaten

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  WB analysis using 68445-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 68445-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 68445-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 68445-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68445-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 68445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 68445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 68445-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 68445-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Jurkat-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, PC-12-Zellen, PC-3-Zellen, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maus-Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68445-1-Ig bindet in WB, IHC, ELISA ULK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ULK1 fusion protein Ag22397
• Vollständiger Name: unc-51-like kinase 1 (C. elegans)
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: NM_003565
• Gene symbol: ULK1
• Gene ID (NCBI): 8408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).