ULK1 Monoklonaler Antikörper

ULK1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D7G1

Kat-Nr. : 68445-1-PBS

Synonyme

1D7G1, ATG1, Autophagy-related protein 1 homolog, EC:2.7.11.1, hATG1

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  WB analysis of PC-3 using 68445-1-Ig (same clone as 68445-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68445-1-Ig (same clone as 68445-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 68445-1-Ig (same clone as 68445-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68445-1-Ig (same clone as 68445-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68445-1-Ig (ULK1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 68445-1-Ig (same clone as 68445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68445-1-Ig (ULK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68445-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68445-1-PBS bindet in WB, IHC, Indirect ELISA ULK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ULK1 fusion protein Ag22397
• Vollständiger Name: unc-51-like kinase 1 (C. elegans)
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: NM_003565
• Gene symbol: ULK1
• Gene ID (NCBI): 8408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ULK1, also named as KIAA0722, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and APG1/unc-51/ULK1 subfamily. It is involved in autophagy. ULK1 is required for autophagosome formation. It forms a stable complex with Atg13 and focal adhesion kinase (FAK) family interacting protein of 200 kDa (FIP 200)(PMID:21460634). ULK1 phosphorylates ATG13/KIAA0652. It is involved in axon growth. ULK1 plays an essential role in neurite extension of cerebellar granule cells. ULK1 represents a potential novel prognostic biomarker for HCC patients and may play an important role during the progression of HCC(PMID:23573318).