UNC13A Polyklonaler Antikörper

UNC13A Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55053-1-PBS

Synonyme

UNC13A/Munc13-1, Munc13-1, KIAA1032, Munc13 1, Protein unc 13 homolog A

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  WB analysis using 55053-1-AP (same clone as 55053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 55053-1-AP (same clone as 55053-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 55053-1-AP (same clone as 55053-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55053-1-AP (UNC13A/Munc13-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 55053-1-AP (same clone as 55053-1-PBS)

  IP result of anti-UNC13A (IP:55053-1-AP, 4ug; Detection:55053-1-AP 1:10000) with mouse brain tissue lysate 1720 ug. This data was developed using the same antibody clone with 55053-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

55053-1-PBS bindet in WB, IP, Indirect ELISA UNC13A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-13 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 193 kDa
• Beobachtetes Molekulargewicht: 193-210 kDa
• GenBank-Zugangsnummer: NM_001080421
• Gene symbol: UNC13A
• Gene ID (NCBI): 23025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.