UNC13A Rekombinanter Antikörper

UNC13A Rekombinant Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240017A6

Kat-Nr. : 83000-5-RR

Synonyme

Munc13-1, Protein unc-13 homolog A, Protein unc 13 homolog A, Munc13 1, KIAA1032

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  WB analysis using 83000-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83000-5-RR (UNC13A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 83000-5-RR

  IP result of anti-UNC13A (IP:83000-5-RR, 4ug; Detection:83000-5-RR 1:1000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse brain using 83000-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83000-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83000-5-RR (UNC13A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83000-5-RR bindet in WB, IHC, IP, ELISA UNC13A und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-13 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 193 kDa
• Beobachtetes Molekulargewicht: 193-210 kDa
• GenBank-Zugangsnummer: NM_001080421
• Gene symbol: UNC13A
• Gene ID (NCBI): 23025
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNC13A, also named KIAA1032, belongs to the unc-13 family. It plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. UNC13A is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). It is essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. UNC13A is also involved in secretory granule priming in INS secretion. The antibody is specific to UNC13A.