UNC5A-Specific Polyklonaler Antikörper

UNC5A-Specific Polyklonal Antikörper für FC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20239-1-AP

Synonyme

KIAA1976, Netrin receptor UNC5A, Protein unc 5 homolog 1, Protein unc 5 homolog A, unc 5 homolog A (C. elegans), UNC5A, UNC5H1

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20239-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20239-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20239-1-AP (UNC5A-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  FC experiment of MCF-7 using 20239-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug UNC5A-Specific antibody (20239-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20239-1-AP bindet in WB, FC,ELISA UNC5A-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-5 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 103 kDa
• GenBank-Zugangsnummer: NM_133369
• Gene symbol: UNC5A
• Gene ID (NCBI): 90249
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNC5A, also named as KIAA1976 and UNC5H1, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5A. It has no cross reaction to UNC5B, UNC5C and UNC5D.