UNC5C-Specific Polyklonaler Antikörper

UNC5C-Specific Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20240-1-PBS

Synonyme

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  WB analysis using 20240-1-AP (same clone as 20240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20240-1-AP (same clone as 20240-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20240-1-AP (same clone as 20240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20240-1-AP (same clone as 20240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20240-1-AP (same clone as 20240-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20240-1-AP (UNC5C-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20240-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20240-1-PBS bindet in WB, IHC, Indirect ELISA UNC5C-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-5 homolog C (C. elegans)
• Berechnetes Molekulargewicht: 103 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 103 kDa
• GenBank-Zugangsnummer: NM_003728
• Gene symbol: UNC5C
• Gene ID (NCBI): 8633
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNC5C, also named as UNC5H3, belongs to the unc-5 family. It is a receptor for netrin required for axon guidance. It mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also involved in corticospinal tract axon guidances independently of DCC. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. The antibody is specific to UNC5C.