UNG Polyklonaler Antikörper

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• KD/KO Validated

UNG Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 12394-1-AP

Synonyme

Uracil-DNA glycosylase, UNG1, EC:3.2.2.27, DGU

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  WB analysis using 12394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12394-1-AP (UNG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 12394-1-AP

  WB result of UNG antibody (12394-1-AP; 1:3000; room temperature for 1.5 hours) with wild-type and UNG knockout HuH-7 cells.

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  IHC staining of mouse heart using 12394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 12394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 12394-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12394-1-AP (UNG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 12394-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12394-1-AP (UNG Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12394-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12394-1-AP (UNG Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12394-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12394-1-AP (UNG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HeLa-Zellen, HuH-7-Zellen
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12394-1-AP bindet in WB, IHC, IF/ICC, ELISA UNG und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UNG fusion protein Ag3072
• Vollständiger Name: uracil-DNA glycosylase
• Berechnetes Molekulargewicht: 304 aa, 34 kDa
• Beobachtetes Molekulargewicht: 28 kDa, 34-40 kDa
• GenBank-Zugangsnummer: BC015205
• Gene symbol: UNG
• Gene ID (NCBI): 7374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UNG(Uracil-DNA glycosylase) removes uracil in DNA resulting from deamination of cytosine or replicative incorporation of dUMP instead of dTMP. Thus, UNG plays a role in suppressing GC-to-AT transition mutations.The UNG gene encodes 2 isoforms that are individually targeted to the mitochondria and the nucleus(PMID:12369930). Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5).

Publikationen

Species	Application	Title
human	WB	Dev Cell; Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates; Authors - Yuan Fu
human	WB	Mol Cell; Uracil processing by SMUG1 in the absence of UNG triggers homologous recombination and selectively kills BRCA1/2-deficient tumors; Authors - Daniele Musiani