UPF1 Polyklonaler Antikörper

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• KD/KO Validated

UPF1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF/ICC, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 23379-1-AP

Synonyme

RENT1, Up-frameshift suppressor 1 homolog, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12

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  WB analysis using 23379-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23379-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 23379-1-AP

  IP result of anti-UPF1 (IP:23379-1-AP, 4ug; Detection:23379-1-AP 1:500) with Raji cells lysate 2000ug.

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  IF Staining of HepG2 using 23379-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UPF1 antibody (23379-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 23379-1-AP

  Immunofluorescent analysis of MCF-7 cells, using UPF1 antibody 23379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Raji-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 3 publications below
• RIP: See 1 publications below

Produktinformation

23379-1-AP bindet in WB, IF/ICC, IP, RIP, ELISA UPF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UPF1 fusion protein Ag16195
• Vollständiger Name: UPF1 regulator of nonsense transcripts homolog (yeast)
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 123-130 kDa
• GenBank-Zugangsnummer: BC039817
• Gene symbol: UPF1
• Gene ID (NCBI): 5976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages; Authors - Teodorus Theo Susanto
human	WB	iScience; Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1; Authors - Lingxiao Tong
human	WB	Breast Cancer Res; Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1.; Authors - Shuiyi Liu
human	WB	Bioengineered; The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells.; Authors - Shizhen Sui
human	WB	J Exp Clin Cancer Res; PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.; Authors - Pingfu Hou
human	WB	Cell Death Dis; PABPC1-induced stabilization of BDNF-AS inhibits malignant progression of glioblastoma cells through STAU1-mediated decay.; Authors - Rui Su