UPF1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UPF1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28384-1-PBS

Synonyme

RENT1, ATP-dependent helicase RENT1, EC:3.6.4.12, KIAA0221, Regulator of nonsense transcripts 1

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  WB analysis using 28384-1-AP (same clone as 28384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28384-1-AP (UPF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 28384-1-AP (same clone as 28384-1-PBS)

  WB result of UPF1 antibody (28384-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells. This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 28384-1-AP (same clone as 28384-1-PBS)

  IP result of anti-UPF1 (IP:28384-1-AP, 4ug; Detection:28384-1-AP 1:800) with HeLa cells lysate 1440 ug. This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 28384-1-AP (same clone as 28384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28384-1-AP (UPF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28384-1-AP (same clone as 28384-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UPF1 antibody (28384-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28384-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28384-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA UPF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UPF1 fusion protein Ag29057
• Vollständiger Name: UPF1 regulator of nonsense transcripts homolog (yeast)
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 123-130 kDa
• GenBank-Zugangsnummer: BC039817
• Gene symbol: UPF1
• Gene ID (NCBI): 5976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.