UPF1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

UPF1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

CloneNo.

3B6B5

Kat-Nr. : 66898-1-Ig

Synonyme

RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1

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  WB analysis using 66898-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66898-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66898-1-Ig

  WB result of UPF1 antibody (66898-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UPF1 transfected HEK-293 cells.

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  WB analysis using 66898-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66898-1-Ig (UPF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66898-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66898-1-Ig (UPF1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66898-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66898-1-Ig (UPF1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66898-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using UPF1 antibody (66898-1-Ig, Clone: 3B6B5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66898-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human UPF1 (66898-1-Ig, Clone:3B6B5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushirngewebe, Maushodengewebe, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• RIP: See 1 publications below

Produktinformation

66898-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), RIP, ELISA UPF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: UPF1 fusion protein Ag28320
• Vollständiger Name: UPF1 regulator of nonsense transcripts homolog (yeast)
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 123-130 kDa
• GenBank-Zugangsnummer: BC039817
• Gene symbol: UPF1
• Gene ID (NCBI): 5976
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

Publikationen

Species	Application	Title
mouse	WB	J Neurosci; Inhibition of Nonsense-Mediated Decay Induces Nociceptive Sensitization through Activation of the Integrated Stress Response; Authors - June Bryan de la Peña
human	WB	Gene; Prenatal diagnostic approaches diagnosed craniosynostosis and identified a novel nonsense variant in SMAD6 in a Chinese fetus; Authors - Zhidan Hong
human	WB	Int J Med Sci; LncRNA SNHG6 promotes proliferation, invasion and migration in colorectal cancer cells by activating TGF-β/Smad signaling pathway via targeting UPF1 and inducing EMT via regulation of ZEB1.; Authors - Xinke Wang
human	WB	Eur J Immunol; The G allele of SNP rs3922 reduces the binding affinity between IGF2BP3 and CXCR5 correlating with a lower antibody production; Authors - Zhaojun Duan ; KD Validated
mouse	WB	J Cell Physiol; Nonsense-mediated mRNA decay suppresses injury-induced muscle regeneration via inhibiting MyoD transcriptional activity; Authors - Yanjie Tan ; KD Validated
	WB	Mol Med; CircMAPK1 induces cell pyroptosis in sepsis-induced lung injury by mediating KDM2B mRNA decay to epigenetically regulate WNK1; Authors - Min Li