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UPF1 Rekombinanter Antikörper

UPF1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3O15

Kat-Nr. : 82791-1-PBS

Synonyme

RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1

Filter:
  • Western Blot (WB) analysis of various lysates using UPF1 Recombinant antibody (82791-1-RR)

    WB analysis using 82791-1-RR (same clone as 82791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82791-1-RR (UPF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UPF1 Recombinant antibody (82791-1-RR)

    IF Staining of HepG2 using 82791-1-RR (same clone as 82791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UPF1 antibody (82791-1-RR, Clone: 3O15 ) at dilution of 1:370 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82791-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82791-1-RR

    Affinity and Kinetic Characterization of 82791-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82791-1-RR against Human UPF1 were performed. The affinity constant is 50.7 nM.

Geprüfte Anwendungen

Produktinformation

82791-1-PBS bindet in WB, IF/ICC, Indirect ELISA UPF1 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen UPF1 fusion protein Ag29057
Vollständiger Name UPF1 regulator of nonsense transcripts homolog (yeast)
Berechnetes Molekulargewicht123 kDa
Beobachtetes Molekulargewicht 123-130 kDa
GenBank-ZugangsnummerBC039817
Gene symbol UPF1
Gene ID (NCBI) 5976
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.