UPF3A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UPF3A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17114-1-AP

Synonyme

Up-frameshift suppressor 3 homolog A, UPF3, RENT3A, HUPF3A, hUpf3

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  WB analysis using 17114-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17114-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17114-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 17114-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 17114-1-AP

  Immunofluorescent analysis of HeLa cells using 17114-1-AP (UPF3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Hodengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

17114-1-AP bindet in WB, IHC, IF/ICC, ELISA UPF3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UPF3A fusion protein Ag10799
• Vollständiger Name: UPF3 regulator of nonsense transcripts homolog A (yeast)
• Berechnetes Molekulargewicht: 443 aa, 51 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC023569
• Gene symbol: UPF3A
• Gene ID (NCBI): 65110
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancer Manag Res; Functions and Clinical Significance of UPF3a Expression in Human Colorectal Cancer.; Authors - Xinmin Bao ; KD Validated
human	WB	Biochim Biophys Acta; Increase of a Group of PTC(+) Transcripts by Curcumin through Inhibition of the NMD Pathway.; Authors - Dairong Feng
human	IHC	J Gastroenterol Hepatol; Genetic compensation response could exist in colorectal cancer: UPF3A up-regulates the oncogenic homologue gene SRSF3 expression corresponding to SRSF6 to promote colorectal cancer metastasis; Authors - Weimin Xu
human	WB	Cells; Co-Inhibition of tGLI1 and GP130 Using FDA-Approved Ketoconazole and Bazedoxifene Is Synergistic Against the Growth and Metastasis of HER2-Enriched and Triple-Negative Breast Cancers; Authors - Sara Manore