UPF3B Polyklonaler Antikörper

UPF3B Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 23301-1-AP

Synonyme

RENT3B, hUpf3B, hUpf3p X, hUpf3p-X, MRXS14

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  WB analysis using 23301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Herzgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

23301-1-AP bindet in WB, IHC, ELISA UPF3B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UPF3B fusion protein Ag18568
• Vollständiger Name: UPF3 regulator of nonsense transcripts homolog B (yeast)
• Berechnetes Molekulargewicht: 483 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC121017
• Gene symbol: UPF3B
• Gene ID (NCBI): 65109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Regulator of nonsense transcripts 3B (UPF3B) is also named as RENT3B, and belongs to the RENT3 family. UPF3B is a core junction protein in the Nonsense-mediated RNA decay (NMD) pathway, involved in the exonuclear transport and quality supervision of mRNA. And UPF3B showed heightened expression in hepatocellular carcinoma (HCC) tissues and was linked with adverse prognosis (PMID: 38889220). UPF3B promotes the dissociation of post-termination ribosomal complexes that lack nascent peptide. UPF3B could promote ribosome recycling, and UPF3B serves as a direct regulator of translation termination (PMID: 29333258).

Publikationen

Species	Application	Title
human	IHC	Front Genet; Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.; Authors - Zhen Zhang