UPK1A Rekombinanter Antikörper

UPK1A Rekombinant Antikörper für WB, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251472C10

Kat-Nr. : 86799-1-PBS

Synonyme

TSPAN21, Tetraspanin-21, Tspan-21, UP1a, UPIa

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  WB analysis using 86799-1-RR (same clone as 86799-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86799-1-RR (UPK1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86799-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse bladder using 86799-1-RR (same clone as 86799-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse bladder tissue using UPK1A antibody (86799-1-RR, Clone: 251472C10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86799-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86799-1-PBS bindet in WB, IF-P, Indirect ELISA UPK1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UPK1A fusion protein Ag17356
• Vollständiger Name: uroplakin 1A
• Berechnetes Molekulargewicht: 258 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC107098
• Gene symbol: UPK1A
• Gene ID (NCBI): 11045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.