UPK1A Rekombinanter Antikörper

UPK1A Rekombinant Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

251472C10

Kat-Nr. : 86799-1-RR

Synonyme

TSPAN21, Tetraspanin-21, Tspan-21, UP1a, UPIa

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  WB analysis using 86799-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86799-1-RR (UPK1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse bladder using 86799-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse bladder tissue using UPK1A antibody (86799-1-RR, Clone: 251472C10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Blasengewebe, Ratten-Blasengewebe
• Erfolgreiche Detektion in IF-P: Maus-Blasengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86799-1-RR bindet in WB, IF-P, ELISA UPK1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: UPK1A fusion protein Ag17356
• Vollständiger Name: uroplakin 1A
• Berechnetes Molekulargewicht: 258 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC107098
• Gene symbol: UPK1A
• Gene ID (NCBI): 11045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin-1a (UPK1A) belongs to the tetraspanin (TM4SF) family. UPK1A plays an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane.