UQCRB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UQCRB Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 10756-1-AP

Synonyme

Complex III subunit 7, Complex III subunit VII, QCR7, QP C, QPC, UQBC, UQBP, UQCRB, UQPC

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Galerie der Validierungsdaten

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  WB analysis using 10756-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10756-1-AP (UQCRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10756-1-AP

  IP Result of anti-UQCRB (IP:10756-1-AP, 3ug; Detection:10756-1-AP 1:600) with mouse heart tissue lysate 3200ug.

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  IHC staining of human breast cancer using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10756-1-AP (UQCRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10756-1-AP (UQCRB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10756-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10756-1-AP (UQCRB Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below

Produktinformation

10756-1-AP bindet in WB, IP, IHC, ELISA UQCRB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UQCRB fusion protein Ag1127
• Vollständiger Name: ubiquinol-cytochrome c reductase binding protein
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC005230
• Gene symbol: UQCRB
• Gene ID (NCBI): 7381
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UQCRB(ubiquinol-cytochrome c reductase binding protein) is also named as UQBP, complex III subunit VII and belongs to the UQCRB/QCR7 family. It is a 13.4 kDa subunit of mitochondrial complex III, plays a crucial role in hypoxia-induced angiogenesis via mitochondrial reactive oxygen species (ROS)-mediated signaling(PMID:21215626). This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Mol Cell; TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III.; Authors - Emanuela Bottani
human	WB	Cell Metab; Mitochondrial complex III ROS regulate adipocyte differentiation.; Authors - Tormos Kathryn V KV
		Sci Adv; Mitochondrial respiration controls the Prox1-Vegfr3 feedback loop during lymphatic endothelial cell fate specification and maintenance.; Authors - Wanshu Ma ; KO Validated
human	WB	Nat Chem Biol; Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly.; Authors - Christopher F Bennett
mouse	WB	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang
mouse	WB	Neurobiol Aging; Up-regulation of key microRNAs, and inverse down-regulation of their predicted oxidative phosphorylation target genes, during aging in mouse brain.; Authors - Li Na N