UQCRQ Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

UQCRQ Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14975-1-AP

Synonyme

Complex III subunit 8, Complex III subunit VIII, QCR8, QP C, QPC, UQCRQ

---

Galerie der Validierungsdaten

• 

  WB analysis using 14975-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14975-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14975-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14975-1-AP

  IP result of anti-UQCRQ (IP:14975-1-AP, 4ug; Detection:14975-1-AP 1:1000) with HepG2 cells lysate 3360 ug.

• 

  IHC staining of human liver cancer using 14975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14975-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14975-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRQ antibody (14975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Lebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below

Produktinformation

14975-1-AP bindet in WB, IP, IHC, IF, ELISA UQCRQ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UQCRQ fusion protein Ag6886
• Vollständiger Name: ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC001390
• Gene symbol: UQCRQ
• Gene ID (NCBI): 27089
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Deficiency of T-Cell Intracellular Antigen 1 in Murine Embryonic Fibroblasts Is Associated with Changes in Mitochondrial Morphology and Respiration.; Authors - Isabel Carrascoso
human	WB	Stem Cells; The Transcription Factor 7-Like 2-Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha Axis Connects Mitochondrial Biogenesis and Metabolic Shift with Stem Cell Commitment to Hepatic Differentiation.; Authors - Anaïs Wanet
rat	WB	Cell Death Dis; Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.; Authors - Yuehong Wang
human		J Biol Chem; Mitochondrial reactive oxygen species regulate transforming growth factor-β signaling.; Authors - Jain Manu M ; KD Validated
mouse	WB	Am J Physiol Endocrinol Metab; Exogenous H2S reduces acetylation levels of mitochondrial respiratory enzymes via regulating NAD+-SIRT3 pathway in cardiac tissues of db/db mice.; Authors - Yu Sun
mouse	WB	Front Endocrinol (Lausanne); The Impaired Bioenergetics of Diabetic Cardiac Microvascular Endothelial Cells.; Authors - Haitao Zhang