UQCRQ Polyklonaler Antikörper

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• KD/KO Validated

UQCRQ Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14975-1-PBS

Synonyme

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  WB analysis using 14975-1-AP (same clone as 14975-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 14975-1-AP (same clone as 14975-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14975-1-AP (UQCRQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

  IP result of anti-UQCRQ (IP:14975-1-AP, 4ug; Detection:14975-1-AP 1:1000) with HepG2 cells lysate 3360 ug. This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14975-1-AP (same clone as 14975-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14975-1-AP (UQCRQ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 14975-1-AP (same clone as 14975-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UQCRQ antibody (14975-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14975-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14975-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA UQCRQ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: UQCRQ fusion protein Ag6886
• Vollständiger Name: ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
• Berechnetes Molekulargewicht: 10 kDa
• Beobachtetes Molekulargewicht: 10 kDa
• GenBank-Zugangsnummer: BC001390
• Gene symbol: UQCRQ
• Gene ID (NCBI): 27089
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.