URAT1 Rekombinanter Antikörper

URAT1 Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230204B3

Kat-Nr. : 82964-1-PBS

Synonyme

SLC22A12, Renal-specific transporter, Renal specific transporter, Organic anion transporter 4-like protein, OATL4

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  WB analysis using 82964-1-RR (same clone as 82964-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82964-1-RR (URAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82964-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82964-1-RR (same clone as 82964-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug URAT1 Recombinant antibody (82964-1-RR, Clone:230204B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82964-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82964-1-PBS bindet in WB, FC (Intra), Indirect ELISA URAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: URAT1 fusion protein Ag27805
• Vollständiger Name: solute carrier family 22 (organic anion/urate transporter), member 12
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC053348
• Gene symbol: URAT1
• Gene ID (NCBI): 116085
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.