USF1 Monoklonaler Antikörper

USF1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C8F1

Kat-Nr. : 67114-1-PBS

Synonyme

bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1

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  WB analysis using 67114-1-Ig (same clone as 67114-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67114-1-Ig (same clone as 67114-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67114-1-Ig (same clone as 67114-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 67114-1-Ig (same clone as 67114-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 67114-1-Ig (same clone as 67114-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67114-1-Ig (same clone as 67114-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67114-1-Ig (same clone as 67114-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67114-1-Ig (USF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67114-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67114-1-PBS bindet in WB, Indirect ELISA USF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USF1 fusion protein Ag17785
• Vollständiger Name: upstream transcription factor 1
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 38-43 kDa
• GenBank-Zugangsnummer: BC035505
• Gene symbol: USF1
• Gene ID (NCBI): 7391
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa