Harmonin Polyklonaler Antikörper

Harmonin Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11358-1-AP

Synonyme

USH1C, Renal carcinoma antigen NY-REN-3, Protein PDZ-73, Autoimmune enteropathy-related antigen AIE-75, Antigen NY-CO-38/NY-CO-37

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  WB analysis using 11358-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11358-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11358-1-AP (Harmonin antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11358-1-AP

  IP result of anti-Harmonin (IP:11358-1-AP, 4ug; Detection:11358-1-AP 1:600) with HEK-293 cells lysate 3000ug.

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  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of Human Colon cancer using 11358-1-AP

  Immunohistochemical analysis of paraffin-embedded Human Colon cancer tissue slide using 11358-1-AP (Harmonin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11358-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Harmonin antibody (11358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, PC-3-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11358-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Harmonin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Harmonin fusion protein Ag1919
• Vollständiger Name: Usher syndrome 1C (autosomal recessive, severe)
• Berechnetes Molekulargewicht: 552 aa, 62 kDa
• Beobachtetes Molekulargewicht: 68-73 kDa
• GenBank-Zugangsnummer: BC016057
• Gene symbol: USH1C/Harmonin
• Gene ID (NCBI): 10083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Harmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B/myosin VIIa and USH1G/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).

Publikationen

Species	Application	Title
mouse	WB,IF	Hum Mol Genet; Impact of the Usher syndrome on olfaction.; Authors - Fabian Jansen
human	IHC	Int J Mol Sci; A Liquid-Liquid Phase Separation-Related Index Associate with Biochemical Recurrence and Tumor Immune Environment of Prostate Cancer Patients; Authors - Qi You