USMG5 Polyklonaler Antikörper

USMG5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17716-1-AP

Synonyme

HCV F transactivated protein 2, HCVFTP2, USMG5

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  WB analysis using 17716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17716-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, humanes Lebergewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

17716-1-AP bindet in WB, IHC, IF, ELISA USMG5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USMG5 fusion protein Ag12086
• Vollständiger Name: up-regulated during skeletal muscle growth 5 homolog (mouse)
• Berechnetes Molekulargewicht: 58 aa, 7 kDa
• Beobachtetes Molekulargewicht: 6 kDa
• GenBank-Zugangsnummer: BC072683
• Gene symbol: USMG5
• Gene ID (NCBI): 84833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice.; Authors - James B Papizan
mouse	IF	Sci Rep; miR-379 links glucocorticoid treatment with mitochondrial response in Duchenne muscular dystrophy.; Authors - M Sanson
rat	WB	Mol Psychiatry; Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala; Authors - Kayla Farrell
human	WB	EBioMedicine; Homozygous variant in translocase of outer mitochondrial membrane 7 leads to metabolic reprogramming and microcephalic osteodysplastic dwarfism with moyamoya disease; Authors - Chia-Yi Li