USP1 Polyklonaler Antikörper

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• KD/KO Validated

USP1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 14346-1-AP

Synonyme

Deubiquitinating enzyme 1, hUBP, ubiquitin specific peptidase 1, Ubiquitin thiolesterase 1, UBP, USP1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 14346-1-AP

  WB result of USP1 antibody (14346-1-AP, 1:1000) with si-Control and si-USP1 transfected HEK293 cells.

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  WB analysis using 14346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14346-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14346-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14346-1-AP

  IP Result of anti-USP1 (IP:14346-1-AP, 3ug; Detection:14346-1-AP 1:300) with HEK-293 cells lysate 3200ug.

• 

  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14346-1-AP (USP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14346-1-AP (USP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human cervical cancer using 14346-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 14346-1-AP (USP1 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 24 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below

Produktinformation

14346-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA USP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP1 fusion protein Ag5686
• Vollständiger Name: ubiquitin specific peptidase 1
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 110 kDa, 100 kDa
• GenBank-Zugangsnummer: BC050525
• Gene symbol: USP1
• Gene ID (NCBI): 7398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. It is also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases (PMID: 9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment (PMID: 18082604).

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.; Authors - Qin Liang
human	WB	Mol Cell; Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.; Authors - Ciccia Alberto A
human	WB,IF	Nat Commun; The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.; Authors - Kyungsoo Ha
human	WB	Nat Commun; hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma.; Authors - Pauline Herviou
human	WB	Cancer Immunol Res; USP22 deubiquitinates CD274 to suppress anti-cancer immunity.; Authors - Xing Huang
human	WB,IHC,IF,IP	Theranostics; CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells; Authors - Apoorvi Tyagi