USP1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

USP1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C10H6

Kat-Nr. : 66069-1-PBS

Synonyme

1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1

---

• 

  WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

  WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66069-1-Ig (same clone as 66069-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66069-1-PBS bindet in WB, IHC, ELISA USP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP1 fusion protein Ag5904
• Vollständiger Name: ubiquitin specific peptidase 1
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC050525
• Gene symbol: USP1
• Gene ID (NCBI): 7398
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).