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USP1 Rekombinanter Antikörper

USP1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251219E10

Kat-Nr. : 86453-1-PBS

Synonyme

Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1, Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment

Filter:
  • Western Blot (WB) analysis of various lysates using USP1 Recombinant antibody (86453-1-RR)

    WB analysis using 86453-1-RR (same clone as 86453-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86453-1-RR (USP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86453-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86453-1-RR

    Affinity and Kinetic Characterization of 86453-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86453-1-RR against Human USP1 were performed. The affinity constant is 14.5 pM.

Geprüfte Anwendungen

Produktinformation

86453-1-PBS bindet in WB, Indirect ELISA USP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen USP1 fusion protein Ag5686
Vollständiger Name ubiquitin specific peptidase 1
Berechnetes Molekulargewicht 88 kDa
Beobachtetes Molekulargewicht 110 kDa, 100 kDa
GenBank-ZugangsnummerBC050525
Gene symbol USP1
Gene ID (NCBI) 7398
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. It is also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases (PMID: 9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment (PMID: 18082604).