USP10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

USP10 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Rezensionen (1)

Kat-Nr. : 19374-1-AP

Synonyme

UBP10, Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12

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  WB analysis using 19374-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19374-1-AP (USP10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 19374-1-AP

  IP result of anti-USP10 (IP:19374-1-AP, 4ug; Detection:19374-1-AP 1:500) with HEK-293 cells lysate 1575 ug.

• 

  IF Staining of HEK-293 using 19374-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP10 antibody (19374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 19374-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using USP10 antibody (19374-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below

Produktinformation

19374-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA USP10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP10 fusion protein Ag7233
• Vollständiger Name: ubiquitin specific peptidase 10
• Berechnetes Molekulargewicht: 87 kDa, 92 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC000263
• Gene symbol: USP10
• Gene ID (NCBI): 9100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

Publikationen

Species	Application	Title
human		Cell; G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.; Authors - Peiguo Yang
	WB	Mol Cell; Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance.; Authors - Anna K Lee
human	WB,IHC,IF,IP	Cell Death Differ; Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma; Authors - Yu-Fei Cao ; KD Validated
human	WB	Nat Commun; Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.; Authors - Hu Lei ; KD Validated
human	IF	J Cell Biol; High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate.; Authors - Brian D Freibaum
mouse	WB	Br J Pharmacol; Limonin stabilises SIRT6 by activating USP10 in cardiac hypertrophy.; Authors - Li-Bo Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gonzalo (Verified Customer) (03-21-2018)

Great antibody for WB and IP

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293

USP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in HEK293 (Cat no:19374-1-AP)