USP10 Monoklonaler Antikörper

USP10 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B10C9

Kat-Nr. : 67917-1-PBS

Synonyme

1B10C9, Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10

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  WB analysis using 67917-1-Ig (same clone as 67917-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67917-1-Ig (USP10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67917-1-Ig (same clone as 67917-1-PBS)

  WB result of USP10 antibody (67917-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP10 transfected MCF-7 cells. This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67917-1-Ig (same clone as 67917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67917-1-Ig (USP10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67917-1-Ig (same clone as 67917-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67917-1-Ig (USP10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67917-1-Ig (same clone as 67917-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using USP10 antibody (67917-1-Ig, Clone: 1B10C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67917-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67917-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA USP10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: USP10 fusion protein Ag7233
• Vollständiger Name: ubiquitin specific peptidase 10
• Berechnetes Molekulargewicht: 87 kDa, 92 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC000263
• Gene symbol: USP10
• Gene ID (NCBI): 9100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP10, a cytoplasmic ubiquitin-specific protease, deubiquitinates p53, reversing Mdm2-induced p53 nuclear export and degradation. After DNA damage, USP10 is stabilized, and a fraction of USP10 translocates to the nucleus to activate p53. The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337. (PMID: 20096447, PMID: 26876938)