USP10 Rekombinanter Antikörper

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• KD/KO Validated

USP10 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240583G6

Kat-Nr. : 81435-2-RR

Synonyme

240583G6, Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10

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  WB analysis using 81435-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 81435-2-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 81435-2-RR

  WB result of USP10 antibody (81435-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP10 transfected MCF-7 cells.

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  IF Staining of HepG2 using 81435-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP10 antibody (81435-2-RR, Clone: 240583G6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, A549-Zellen, HEK-293-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81435-2-RR bindet in WB, IF/ICC, ELISA USP10 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: USP10 fusion protein Ag7233
• Vollständiger Name: ubiquitin specific peptidase 10
• Berechnetes Molekulargewicht: 87 kDa, 92 kDa
• Beobachtetes Molekulargewicht: 100-130 kDa
• GenBank-Zugangsnummer: BC000263
• Gene symbol: USP10
• Gene ID (NCBI): 9100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.