USP11 Polyklonaler Antikörper

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• KD/KO Validated

USP11 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10244-1-AP

Synonyme

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 10244-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 10244-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10244-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10244-1-AP

  IP Result of anti-USP11 (IP:10244-1-AP, 4ug; Detection:10244-1-AP 1:300) with HEK-293 cells lysate 1480ug.

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  IHC staining of human colon cancer using 10244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10244-1-AP (USP11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, humanes Hodengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 4 publications below

Produktinformation

10244-1-AP bindet in WB, IP, IHC, ELISA USP11 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: USP11 fusion protein Ag0296
• Vollständiger Name: ubiquitin specific peptidase 11
• Berechnetes Molekulargewicht: 963 aa, 110 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC000350
• Gene symbol: USP11
• Gene ID (NCBI): 8237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair.; Authors - Marek Adamowicz
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	WB,IHC	Clin Transl Med; EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.; Authors - Hongyu Zhao
human	IHC	Oncogene; The deubiquitinase USP11 regulates cell proliferation and ferroptotic cell death via stabilization of NRF2 USP11 deubiquitinates and stabilizes NRF2.; Authors - Chunjie Meng
human	WB,IHC	Cancer Lett; The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway.; Authors - Lijun Qiao ; KD Validated
human	WB	Oncotarget; Ubiquitin-specific protease 11 functions as a tumor suppressor by modulating Mgl-1 protein to regulate cancer cell growth.; Authors - Key-Hwan Lim ; KD Validated